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Updated: Apr 16, 2026
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Published on: April 6, 2022
Ex vivo optical measurements of glucose diffusion kinetics in native and diabetic mouse skin
Daria K Tuchina1, Rui Shi, Alexey N Bashkatov
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China; Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia. tuchinadk@mail.ru.
Abstract:
The aim of this study was to estimate the glucose diffusion coefficients ex vivo in skin of mice with diabetes induced in vivo by alloxan in comparison to non-diabetic mice. The temporal dependences of collimated transmittance of tissue samples immersed in glucose solutions were measured in the VIS-NIR spectral range to quantify the glucose diffusion/permeability coefficients and optical clearing efficiency of mouse skin. The average thickness of intact healthy and diabetic skin was 0.023 ± 0.006 cm and 0.019 ± 0.005 cm, respectively. Considerable differences in optical and kinetic properties of diabetic and non-diabetic skin were found: clearing efficiency was 1.5-fold better and glucose diffusivity was 2-fold slower for diabetic skin. Experimental Setup for measuring collimated transmittance spectra of mouse skin samples.
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