Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

6.1K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
6.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable and label-free optical detection of sweat glucose using functionalized plasmonic nanopillar array.

Microsystems & nanoengineering·2026
Same author

Fe-doped nanodiamond-based photo-Fenton catalyst for dual-modal fluorescence imaging and improved chemotherapeutic efficacy against tumor hypoxia.

RSC advances·2024
Same author

Metabolic patterns of sweat-extracellular vesicles during exercise and recovery states using clinical grade patches.

Frontiers in physiology·2023
Same author

Precise Sn-Doping Modulation for Optimizing CdWO<sub>4</sub> Nanorod Photoluminescence.

International journal of molecular sciences·2022
Same author

Multimodal bioimaging using nanodiamond and gold hybrid nanoparticles.

Scientific reports·2022
Same author

Raman Spectroscopic Study of TiO<sub>2</sub> Nanoparticles' Effects on the Hemoglobin State in Individual Red Blood Cells.

Materials (Basel, Switzerland)·2021

Related Experiment Video

Updated: Apr 16, 2026

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
14:33

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Published on: April 8, 2022

4.2K

Overview of single-cell elastic light scattering techniques.

Matti Kinnunen1, Artashes Karmenyan2

  • 1University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, 90014 Oulu, Finland.

Journal of Biomedical Optics
|March 12, 2015
PubMed
Summary

Modern optical methods using elastic light scattering (ELS) offer advanced single-cell analysis in life sciences. These techniques, including Fourier transform light scattering (FTLS), enable detailed study of cellular dynamics and biological systems.

More Related Videos

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.7K
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.1K

Related Experiment Videos

Last Updated: Apr 16, 2026

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
14:33

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Published on: April 8, 2022

4.2K
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.7K
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.1K

Area of Science:

  • Biophysics
  • Optical Physics
  • Cell Biology

Background:

  • Elastic light scattering (ELS) is a powerful optical technique with diverse applications in biomedicine and life sciences.
  • Recent advancements in experimental systems have enhanced the utility of ELS for single-cell analysis.
  • Understanding cellular structures and dynamics is crucial for advancements in biological research and medical diagnostics.

Purpose of the Study:

  • To review modern optical methods based on elastic light scattering (ELS) for single-cell analysis.
  • To discuss the technical features and biomedical applications of various ELS techniques.
  • To explore new directions and potential applications of ELS in life sciences, focusing on single-cell investigations.

Main Methods:

  • Elastic scattering spectroscopy
  • Optical tweezer-assisted measurements
  • Goniometers
  • Fourier transform light scattering (FTLS)
  • Microscopic methods

Main Results:

  • FTLS significantly enhances single-cell analysis by enabling monitoring of dynamic changes.
  • Goniometric measurements of ELS from optically trapped single cells provide valuable data.
  • The review highlights the integration of theoretical modeling with experimental verification for ELS in biological systems.

Conclusions:

  • ELS methods, particularly FTLS, offer significant potential for detailed single-cell analysis and monitoring of cellular dynamics.
  • Experimental verification of theoretical models is crucial for advancing ELS applications in biomedicine.
  • Continued development of ELS techniques promises to expand their utility in life sciences and biomedical research.