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Genome sequence of a clinical isolate of dermatophyte, Trichophyton rubrum from India

Chitra Latka1, Sanchita Sanchaya Dey1, Siddharth Mahajan2

  • 1Genome Informatics and Structural Biology Unit, CSIR-Institute of Genomics and Integrative Biology, New Delhi 110020, India Academy of Scientific and Innovative Research, New Delhi 110025, India.

FEMS Microbiology Letters
|March 13, 2015
PubMed

Insights

We sequenced the genome of Trichophyton rubrum var. raubitschekii, a fungus causing skin infections. This study aids in understanding fungal diversity and identifying virulence factors for treating dermatophytosis.

Area of Science:

  • Medical Mycology
  • Genomics
  • Infectious Diseases

Background:

  • Trichophyton rubrum is a primary cause of human dermatophytosis globally.
  • Onychomycosis is a common fungal infection of the nails.

Purpose of the Study:

  • To report the draft genome sequence of T. rubrum var. raubitschekii from India.
  • To analyze virulence factors, specifically secreted subtilases, in this dermatophyte.

Main Methods:

  • Whole-genome sequencing of a clinical isolate of T. rubrum var. raubitschekii.
  • Bioinformatic analysis of predicted protein-coding sequences, tRNA, and rRNA genes.
  • Sequence analysis of secreted subtilases to identify distinct subfamilies.

Main Results:

  • The draft genome size is 25.2 MB, with 8265 predicted protein-coding sequences.
  • Analysis revealed three distinct subfamilies of secreted subtilases, key virulence factors.
  • The isolate was obtained from a patient with onychomycosis in Delhi, India.

Conclusions:

  • The genome sequence provides a foundation for understanding dermatophyte diversity.
  • This data will help identify virulence factors and elucidate pathogenesis mechanisms.
  • Further research can leverage this genomic information for targeted antifungal strategies.
Keywords:
Trichophyton rubrum var. raubitschekiidermatophytosisfilamentous funginext-generation sequencingsubtilisin proteases

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