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Updated: Apr 16, 2026

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Published on: July 6, 2022
Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots
Emily J Sylak-Glassman1,2,3, Julia Zaks2,4,5, Kapil Amarnath1,2,6
1Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA.
Abstract:
We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.
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