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Updated: Apr 16, 2026
Terminal H-reflex Measurements in Mice
Published on: June 16, 2022
Between-day reliability of the trapezius muscle H-reflex and M-wave
Steffen Vangsgaard1, Ernst A Hansen1, Pascal Madeleine1
1Physical Activity and Human Performance group, SMI, Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 7 D-3, DK-9220, Aalborg, Denmark.
Introduction:
The aim of this study was to investigate the between-day reliability of the trapezius muscle H-reflex and M-wave.
Methods:
Sixteen healthy subjects were studied on 2 consecutive days. Trapezius muscle H-reflexes were evoked by electrical stimulation of the C3/4 cervical nerves; M-waves were evoked by electrical stimulation of the accessory nerve. Relative reliability was estimated by intraclass correlation coefficients (ICC2,1 ). Absolute reliability was estimated by computing the standard error of measurement (SEM) and the smallest real difference (SRD). Bland-Altman plots were constructed to detect any systematic bias.
Results:
Variables showed substantial to excellent relative reliability (ICC = 0.70-0.99). The relative SEM ranged from 1.4% to 34.8%; relative SRD ranged from 3.8% to 96.5%. No systematic bias was present in the data.
Conclusions:
The amplitude and latency of the trapezius muscle H-reflex and M-wave in healthy young subjects can be measured reliably across days.
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