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Silver decahedral nanoparticles-enhanced fluorescence resonance energy transfer sensor for specific cell imaging
Hui Li1, Hongting Hu1, Danke Xu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China.
Analytical Chemistry
|March 13, 2015
Summary
We developed a novel silver nanocluster sensor for fluorescence imaging of leukemia cells. This sensor enhances fluorescence detection of PTK-7 on CCRF-CEM cells, offering high sensitivity and specificity.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Fluorescence resonance energy transfer (FRET) sensors are valuable tools for biological imaging.
- Targeted imaging of specific cell types, like leukemia cells, requires highly sensitive and specific detection methods.
- Silver nanoclusters offer unique optical properties that can enhance sensor performance.
Purpose of the Study:
- To develop and evaluate a novel silver decahedral nanoparticles (Ag10NPs)-based FRET sensor for the fluorescence imaging of CCRF-CEM cells.
- To investigate the role of Ag10NPs in enhancing sensor performance and specificity.
- To assess the sensor's sensitivity and specificity for detecting membrane protein tyrosine kinase-7 (PTK-7) on leukemia cells.
Main Methods:
- Fabrication of a FRET sensor by functionalizing Ag10NPs with aptamers (Sgc8-FITC) and quencher strands (BHQ-1).
- Utilizing the sensor for fluorescence imaging of CCRF-CEM cells, a human acute lymphoblastic leukemia cell line.
- Analyzing the FRET mechanism, including aptamer binding, quencher displacement, and fluorescence enhancement upon target cell interaction.
Main Results:
- The Ag10NPs-based FRET sensor (Ag10-Sgc8-F/Q) successfully achieved fluorescence imaging of CCRF-CEM cells.
- Binding of the sensor to CCRF-CEM cells led to BHQ-1 displacement, disrupting FRET and enhancing FITC fluorescence.
- Ag10NPs significantly amplified FITC fluorescence intensity, improving overall sensor performance.
- The developed sensor demonstrated superior sensitivity and specificity compared to bare FRET sensors.
Conclusions:
- Ag10NPs-based FRET sensors represent a promising platform for sensitive and specific cellular imaging.
- This sensor technology holds potential for the early detection and diagnosis of leukemia.
- The enhanced fluorescence properties of Ag10NPs contribute to improved imaging capabilities for targeted cell detection.
