[Determination of olanzapine in cadaveric blood]

The authors present the results of experimental isolation of olanzapine from the biological objects in conjunction with the detailed description of the method for olanzapine extraction from cadaveric blood with the use of one of the amphiphilic solvents (e.g. acetone), purification by solvent extraction, and TLC-screening. Olanzapine was quantitatively determined by UV-spectrophotometry following its chromatography and elution. It was shown that one-step extraction from cadaveric blood by the proposed method made it possible to detect 55% of the total olanzapine content. The method was validated in the studies of blood and liver from the laboratory animals (rats) and can be recommended for the investigation in a chemical toxicological laboratory.