Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 16, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.9K

Robust high-throughput batch screening method in 384-well format with optical in-line resin quantification.

Jörg Kittelmann1, Marcel Ottens2, Jürgen Hubbuch1

  • 1Section IV: Biomolecular Separation Engineering, Institute of Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 1, 76131 Karlsruhe, Germany.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|March 14, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same author

Reply to comments on "Comprehensive stress study on recombinant adeno-associated virus vectors: Evaluating the capabilities of established analytical techniques".

Journal of pharmaceutical sciences·2026
Same author

Optimizing single molecule, real-time sequencing for enhanced characterization of adeno-associated viral vector genomes.

Molecular therapy. Advances·2026
Same author

Proof of concept for aqueous two-phase system-based extraction of cell-free DNA from plasma for liquid biopsy applications.

Scientific reports·2026
Same author

Comparing isotherm parameter determination methods for hydrophobic interaction chromatography.

Journal of chromatography. A·2026
Same author

Comprehensive stress study on recombinant adeno-associated virus vectors: Evaluating the capabilities of established analytical techniques.

Journal of pharmaceutical sciences·2026
This summary is machine-generated.

A new optical method enables resin quantification for high-throughput screening (HTS) in 384-well plates. This advances protein-adsorbent interaction studies, offering increased efficiency and data quality in downstream process development.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Analytical Chemistry

Background:

  • High-throughput batch screening is crucial for downstream process development.
  • Current methods in 96-well formats are limited by sample consumption and throughput.
  • No established screening process exists for the 384-well microplate format.

Purpose of the Study:

  • Introduce a novel in-well resin quantification method for 384-well microplates.
  • Develop a high-throughput screening (HTS) batch isotherm process for 384-well format.
  • Enable screening of isotherm parameters with reduced sample and time.

Main Methods:

  • Developed an optical method for in-well resin quantification.
  • Utilized resin volumes as low as 0.1 μL in 384-well microplates.
Keywords:
Batch isothermBootstrap analysisHigh-throughput screening (HTS)Liquid handling station (LHS)Monte CarloResin quantification

More Related Videos

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.1K
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

11.1K

Related Experiment Videos

Last Updated: Apr 16, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.9K
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.1K
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

11.1K
  • Integrated optical sample volume quantification for isotherm parameter screening.

    • Main Results:

    • Successfully implemented an HTS batch isotherm process in the 384-well format.
    • Quantified resin volumes optically with high precision.
    • Validated results using bootstrap analysis and Monte Carlo error propagation studies.

    Conclusions:

    • The new method facilitates screening processes in the 384-well format on HTS stations.
    • This approach enhances screening data quality and significantly increases throughput.
    • Advances protein-adsorbent interaction studies and downstream process development.