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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Apr 16, 2026

Mouse Genome Engineering Using Designer Nucleases
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[Genome editing with programmable site-specific nucleases].

Takashi Yamamoto1, Naoaki Sakamoto, Tetsushi Sakuma

  • 1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University.

Uirusu
|March 14, 2015
PubMed
Summary
This summary is machine-generated.

Genome editing utilizes programmable nucleases like CRISPR/Cas9 to modify genes. This technology facilitates the creation of disease models in cells and animals, advancing genetic research.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • Genome editing technologies, including TALENs and CRISPR/Cas9, have revolutionized biological research.
  • The ability to precisely modify DNA sequences has enabled new approaches to studying gene function and disease mechanisms.

Purpose:

  • This review introduces the fundamental principles of genome editing using programmable nucleases.
  • It also provides an overview of the current landscape and applications of this transformative technology.

Summary:

  • Genome editing employs site-specific nucleases to alter target genes with high precision.
  • Programmable nucleases, such as Transcription Activator-Like Effector Nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, are key tools in this field.
  • The development of these technologies has significantly advanced the creation of cellular and animal models for human diseases.

Impact:

  • Genome editing accelerates the development of disease models, aiding in the study of complex human conditions.
  • It offers unprecedented capabilities for genetic manipulation in previously challenging cell types and organisms.
  • This technology is crucial for advancing genetic research and therapeutic development.