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Updated: Apr 16, 2026

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
Hymenobacter wooponensis sp. nov., isolated from wetland freshwater
Joo Won Kang1, Ji Hee Lee1, Keun Sik Baik2
1Department of Biology, College of life science and Natural Resources, Sunchon National University, Suncheon 540-950, Republic of Korea.
Abstract:
A non-motile and rod-shaped bacterium, designated strain WM78T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15:0, anteiso-C15:0, C16:1ω7c and/or C16:1ω6c and iso-C17:1 I and/or anteiso-C17:1 B. The strain contained MK-7 as the major isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The genomic DNA G+C content was 62 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WM78T forms evolutionary lineage within the radiation comprising members of the genus Hymenobacter with Hymenobacter gelipurpurascens Txg1T (97.7 % sequence similarity) as its nearest neighbour. A number of phenotypic characteristics distinguished strain WM78T from related members of the genus Hymenobacter. The evidence presented in this study support the designation of strain WM78T as a representative of a novel species in the genus Hymenobacter for which the name Hymenbactor wooponensis sp. nov. is proposed. The type strain is WM78T ( = KCTC 32528T = JCM 19491T).
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