Related Experiment Video
Updated: Apr 16, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
A new DNA-intercalative cytotoxic allylic xanthone from Swertia corymbosa
Chokkalingam Uvarani1, Karuppannan Arumugasamy, Kumarasamy Chandraprakash
1Department of Chemistry, School of Chemical Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India (phone: +91 422 2428314; fax: +91 4222422 387); Department of Chemistry, Richardson College for the Environmental and Science Complex, The University of Winnipeg, 599 Portage Avenue, Winnipeg Manitoba, R3B 2G3 Canada, (phone: +1-204-7869389; fax: +1-204-7742401).
Abstract:
Phytochemical investigation of the CHCl3 fraction of Swertia corymbosa resulted in the isolation of a new 3-allyl-2,8-dihydroxy-1,6-dimethoxy-9H-xanthen-9-one (1), along with four known xanthones, gentiacaulein (3), norswertianin (4), 1,3,6,8-tetrahydroxyxanthone (5), and 1,3-dihydroxyxanthone (6). Structure of compound 1 was elucidated with the aid of IR, UV, NMR, and MS data, and chemical transformation via new allyloxy xanthone derivative (2). Compounds 1-6 exhibited various levels of antioxidant and anti-α-glucosidase activities. Absorption and fluorescence spectroscopic studies on 1-6 indicated that these compounds could interact with calf thymus DNA (CT-DNA) through intercalation and with bovine serum albumin (BSA) in a static quenching process. Compound 1 was found to be significantly cytotoxic against human cancer cell lines HeLa, HCT116, and AGS, and weakly active against normal NIH 3T3 cell line.
Related Concept Videos
Inhibitors of Bacterial DNA Synthesis
Aryldiazonium Salts to Azo Dyes: Diazo Coupling

