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Hypervariable polymorphism of autosomal origin detected by the Y-chromosome derived probe, pHY10
Jinrui Idengaku Zasshi. the Japanese Journal of Human Genetics
|September 1, 1989
Summary
A DNA probe (pHY10), typically used for Y chromosome detection, unexpectedly revealed a highly variable genetic marker on autosomal DNA. This finding offers new potential for forensic and human genetic analysis.
Area of Science:
- Human Genetics
- Molecular Biology
- Forensic Science
Background:
- The pHY10 recombinant DNA probe specifically hybridizes to the DYZ1 family on the human Y chromosome.
- This probe is commonly utilized for Y chromosome detection due to its widespread use and availability.
Purpose of the Study:
- To investigate the utility of the pHY10 probe in analyzing human genomic DNA.
- To identify novel polymorphic markers for genetic analysis.
Main Methods:
- Human genomic DNA was digested with restriction enzymes.
- The digested DNA was probed with the recombinant DNA probe pHY10.
- Restriction fragment length polymorphism (RFLP) analysis was performed.
Main Results:
- The pHY10 probe detected a hypervariable polymorphism in autosomal DNA when digested with TaqI.
- No polymorphisms were observed with 12 other restriction enzymes.
- Codominant segregation of the identified autosomal polymorphism was confirmed through family studies.
Conclusions:
- The pHY10 probe, beyond its Y chromosome applications, identifies a valuable autosomal polymorphic marker.
- This autosomal polymorphism exhibits high discrimination potential.
- The probe's characteristics make it suitable for forensic and human genetic applications.