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Published on: July 20, 2016
Changed expression and function of P-gp in peripheral blood CD56 + cells predicting chemoresistance in non-Hodgkin
Li Han1, Xiaojuan Guo1, Hua Bian1
1Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan, China.
Background:
Multi-drug resistance (MDR) remains to be a major obstacle toward successful chemotherapy of NHL patients. P-glycoprotein (P-gp), a classical protein associated with MDR, has been observed in peripheral blood CD56 + cells with high expression and activity. While the CD56 expression has been shown to be associated with a highly aggressive clinical course and chemoresistance in Non-Hodgkin lymphoma (NHL).
Objective:
To investigate the role of peripheral blood CD56+ cells in predicting the MDR of NHL by determining the P-gp expression and function of the CD56+ cells.
Methods:
The expression levels of MDR1 mRNA and MRP1 mRNA and the function of P-gp in the CD56+ cells were evaluated by RT-qPCR and flow cytometry respectively in 52 chemoresistant and 47 chemosensitive NHL patients and 48 healthy donors.
Results:
In the chemoresistant group, the mRNA expression level of MDR1 elevated about 2∼8 fold (mean = 4.24 ± 0.17) in the purified CD56+ cells, whereas there was only about 1∼2.5 fold (mean = 1.69 ± 0.41) elevated for the MRP1 gene. The mean fold change of MDR1 mRNA expression in the chemoresistant group significantly increased when compared with that in the chemosensitive patients (P < 0.001). The mean fluorescence intensities (MFI) in the total gated CD56+ and Rho123 double positive cells in the chemoresistant patients statistically decreased compared with that in the healthy controls and the chemosensitive NHL patients (P < 0.01).
Conclusions:
Determining the P-gp expression and function of the peripheral blood CD56+ cells may help predict the MDR of NHL, thus has profound guiding significance for NHL treatment.
