Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode.

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biomedical Engineering

Background:

• Superoxide anion radical (O2(●-)) is a key reactive oxygen species implicated in various physiological and pathological processes.
• Accurate detection of O2(●-) is crucial for understanding oxidative stress and related diseases.
• Existing detection methods often suffer from limitations in sensitivity, selectivity, or stability.

Purpose of the Study:

• To develop a high-performance enzyme-based electrochemical sensor for sensitive and selective detection of superoxide anion radical (O2(●-)).
• To utilize a novel composite material for enhanced electrochemical sensing performance.

Main Methods:

• Synthesis of platinum-palladium nanoparticles (PtPd NPs) on polydopamine (PDA)-coated reduced graphene oxide (RGO).
• Characterization of the PtPd-PDA-RGO composite using various microscopy and spectroscopy techniques (TEM, SEM, FTIR, XRD, XPS).
• Immobilization of superoxide dismutase (SOD) onto the PtPd-PDA-RGO composite to create the electrochemical sensor.

Main Results:

• The PtPd-PDA-RGO composite was successfully synthesized and characterized.
• The fabricated SOD/PtPd-PDA-RGO sensor demonstrated high sensitivity (909.7 μA mM(-1) cm(-2)) for O2(●-) detection.
• The sensor exhibited a wide linear range (0.016–0.24 mM), low detection limit (2 μM), excellent selectivity, reproducibility, and stability.

Conclusions:

• The developed enzyme-based electrochemical sensor based on the PtPd-PDA-RGO composite shows significant potential for O2(●-) detection.
• The synergistic effects of PDA and PtPd NPs contribute to the enhanced sensing performance.
• This sensor offers a promising platform for applications in oxidative stress monitoring and diagnostics.