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Liquid-liquid extraction and liquid chromatography-mass spectrometry detection of curcuminoids from bacterial culture
Suryani Tan1, Thusitha W T Rupasinghe2, Dedreia L Tull2
1CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia; Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Vic 3010, Australia; Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia.
Abstract:
Liquid chromatography-mass spectrometry (LC-MS) has been used to detect polyphenolic curcuminoids found in turmeric but studies of metabolism by bacterial and mammalian cells in vitro are compromised by poor recovery from the culture medium. We report a liquid-liquid extraction procedure with ethyl acetate and use LC-MS to quantify extracted curcuminoids. Ethyl acetate allows recoveries of ∼ 80-86% of curcuminoids from the bacterial growth medium, bacterial cell lysate and combined bacterial cell and growth medium matrices; a clear improvement over acetonitrile where recoveries were ∼ 25-66%. This optimised method will enable studies of curcuminoid metabolism and may be applicable to other hydrophobic polyphenolic compounds.
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