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Variation among Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks collected from cattle across Thailand.
S Kaewmongkol1, G Kaewmongkol, N Inthong
1Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom, Thailand.
Experimental & Applied Acarology
|March 18, 2015
Summary
Investigating Bm86 variation in Thai cattle ticks revealed genetic differences linked to vaccine effectiveness. This polymorphism suggests distinct Thai and Latin American tick groups, impacting anti-tick vaccine strategies.
Area of Science:
- Veterinary Entomology
- Molecular Phylogenetics
- Vaccine Development
Background:
- Recombinant Bm86 and Bm95 vaccines offer sustainable alternatives to chemical pesticides for cattle tick control.
- Geographical Bm86 polymorphism may reduce the efficacy of existing anti-tick vaccines.
- Understanding tick genetic diversity is crucial for optimizing vaccine performance.
Purpose of the Study:
- To investigate the genetic variation of Bm86 in cattle ticks across different regions of Thailand.
- To analyze the phylogenetic relationships of Thai cattle tick Bm86 variants.
- To assess potential implications for the efficacy of Bm86-based anti-tick vaccines.
Main Methods:
- Sequencing of Bm86 cDNA and deduced amino acid sequences from Thai cattle tick samples.
- Phylogenetic analysis of Bm86 variants to identify geographical groupings.
- Morphological characterization of Thai female and male cattle ticks.
Main Results:
- Thai Bm86 sequences showed 95.6-97.0% nucleotide and 91.5-93.5% amino acid identity to the Australian reference strain.
- Phylogenetic analysis identified two distinct groups: a Thai group and a Latin American group.
- Morphological analysis revealed Rhipicephalus (Boophilus) microplus-like females and Rhipicephalus (Boophilus) australis-like males, with some exhibiting mixed features.
Conclusions:
- Significant Bm86 polymorphism exists among Thai cattle ticks, forming distinct phylogenetic groups.
- The observed genetic and morphological variations may impact the effectiveness of current Bm86-based vaccines.
- Further research is needed to evaluate vaccine efficacy against Thai tick strains and to clarify Thai cattle tick phylogeny.
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