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Updated: Aug 6, 2026
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
miR-150 is a factor of survival in prostate cancer patients
Liu Dezhong1, Zhang Xiaoyi, Long Xianlian
1Department of Urology, Second Artillery General Hospital of PLA, Beijing, China.
Purpose:
Prostate cancer (PC) is the most common malignant disease in males and the second leading cause of cancer related deaths in men in developed countries. The purpose of this study was to investigate whether microRNA (miR)-150 is a factor influencing survival in prostate cancer patients.
Methods:
miR-150 mRNA and protein expression levels in prostatic cancer cell lines and healthy tissues were determined by quantitative (q) RT-PCR and Western blotting. Additionally, the protein expression of miR-150 was detected by immunohistochemistry.
Results:
High miR-150 expression was positively correlated with tumor recurrence or metastasis (p=0.010). In addition, PC patients with high miR-150 expression had significantly poorer overall survival/OR (hazard ratio/HR, 1.87; 95% confidence interval/CI, 1.19-2.94; p=0.006) and poorer disease-free survival/DFS (HR, 1.90; 95% CI, 1.21- 2.98; p=0.005) than those with low miR-150 expression. The cumulative 5-year OS was only 35.19% (95% CI, 26.18- 44.20) in the high miR-150 expression group, whereas it was 55.93% (95% CI, 43.26-68.60) in the low miR-150 expression group (p<0.05). Multivariate Cox regression analysis demonstrated that the expression of miR-150, tumor size, and number of tumor lesions were independent prognostic predictors for OS in PC patients.
Conclusion:
miR-150 was overexpressed in PC at both the mRNA and protein levels, and high expression of miR-150 could serve as a novel and reliable prognostic biomarker for PC patients.
Insights
High microRNA-150 (miR-150) expression in prostate cancer (PC) correlates with poorer survival and recurrence. This finding suggests miR-150 may be a novel prognostic biomarker for PC patients, aiding in risk stratification.
Area of Science:
- Oncology
- Molecular Biology
- Biomarker Discovery
Background:
- Prostate cancer (PC) is a leading cause of cancer death in men.
- Identifying reliable prognostic biomarkers is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the role of microRNA (miR)-150 in prostate cancer.
- To determine if miR-150 expression levels correlate with patient survival and disease progression.
Main Methods:
- Quantitative RT-PCR and Western blotting were used to measure miR-150 mRNA and protein expression in PC cell lines and tissues.
- Immunohistochemistry was employed to detect miR-150 protein expression.
- Survival analysis, including overall survival (OS) and disease-free survival (DFS), was performed.
Main Results:
- miR-150 was overexpressed in PC tissues compared to healthy tissues.
- High miR-150 expression was significantly associated with tumor recurrence and metastasis (p=0.010).
- Patients with high miR-150 expression exhibited significantly poorer OS (HR=1.87) and DFS (HR=1.90) compared to those with low expression.
- Multivariate analysis identified miR-150 expression as an independent prognostic predictor for OS.
Conclusions:
- Overexpression of miR-150 at both mRNA and protein levels is observed in prostate cancer.
- High miR-150 expression is a reliable prognostic biomarker for predicting survival in PC patients.
- miR-150 holds potential as a novel biomarker for risk stratification and personalized treatment strategies in prostate cancer.
