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Updated: Apr 16, 2026

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Published on: December 4, 2015
Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013
Mariette F Ducatez1, Félix Awoume2, Richard J Webby3
1INRA, UMR 1225, IHAP, F-31076 Toulouse, France; Université de Toulouse, INP, ENVT, UMR 1225, IHAP, F-31076 Toulouse, France.
Abstract:
We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lomé in Togo. Influenza A virus genome was detected by RT-PCR in 2.5-12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eight genes similar to A(H1N1)pdm09 virus genes circulating in humans in 2012-2013, suggesting human-to-swine transmission of the pathogen.
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