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Related Experiment Videos

Mitogenic factors in prostatic tissue and expressed prostatic secretion.

R E Tackett, W D Heston, R F Parrish

    The Journal of Urology
    |January 1, 1985
    PubMed
    Summary

    Benign prostatic hyperplasia (BPH) tissue and secretions contain growth factors that stimulate fibroblast proliferation. A high molecular weight factor unique to BPH may play a role in its development.

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    Area of Science:

    • Urology
    • Cell Biology
    • Biochemistry

    Background:

    • Benign prostatic hyperplasia (BPH) is a common condition in aging men.
    • The pathogenesis of BPH is not fully understood but likely involves hormonal and growth factor influences.

    Purpose of the Study:

    • To characterize polypeptide growth factors in expressed prostatic secretions (EPS) and BPH tissue extracts.
    • To investigate the potential role of these factors in BPH development.

    Main Methods:

    • Mitogenic activity was assessed using tritium-labeled thymidine uptake in cultured 3T3 fibroblasts.
    • Gel filtration chromatography was used to estimate molecular weights of active fractions.
    • Bioassays quantified mitogenic activity per milligram of protein.

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    Main Results:

    • EPS and BPH tissue extracts exhibited mitogenic activity.
    • Molecular weights of mitogenic fractions ranged from 30,000 to 300,000 daltons.
    • A high molecular weight component (300,000 daltons) was present in BPH tissue extracts but not in normal prostate tissue.
    • A low molecular weight inhibitor of fibroblast growth was detected in both normal and BPH samples.

    Conclusions:

    • Prostatic growth factors are present in EPS and BPH tissue.
    • A specific high molecular weight growth factor may be implicated in the pathogenesis of BPH.
    • Further research is needed to elucidate the precise roles of these factors in BPH.