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LATS2 induced by TNF-alpha and inhibited cell proliferation and invasion by phosphorylating YAP in oral squamous cell

Cong Dong1,2, Kui-Jie Wei3, Wen-Bin Zhang1

  • 1Shanghai Key Laboratory of Stomatology, Department of Oral and Cranio-Maxillofacial Science, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
|March 19, 2015
PubMed
Abstract

Insights

Large tumor suppressor 2 (LATS2) inhibits oral squamous cell carcinoma (OSCC) progression by phosphorylating YAP. Tumor necrosis factor-alpha (TNF-α) induces LATS2, suggesting LATS2 as a potential therapeutic target for OSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Large tumor suppressor 2 (LATS2) is a known Hippo pathway component that phosphorylates and inactivates YAP, acting as a tumor suppressor in various human cancers.
  • The specific role and regulatory mechanisms of LATS2 in oral squamous cell carcinoma (OSCC) remain underexplored.

Purpose of the Study:

  • To investigate the role of LATS2 in OSCC.
  • To elucidate the mechanisms regulating LATS2 expression in OSCC.
  • To determine the functional impact of LATS2 on OSCC cell behavior.

Main Methods:

  • Western blotting to detect LATS2 and phosphorylated YAP expression in HN6 cells treated with TNF-α.
  • Luciferase reporter assays to confirm YAP phosphorylation by LATS2.
  • Assays for cell proliferation, anchorage-independent growth, invasion, and in vivo xenograft growth to assess the effects of LATS2 overexpression.

Main Results:

  • LATS2 was confirmed to phosphorylate YAP.
  • TNF-α dose- and time-dependently induced LATS2 expression in HN6 cells.
  • Overexpression of LATS2 significantly inhibited OSCC cell proliferation, colony formation, invasion, and in vivo tumor growth.

Conclusions:

  • LATS2, induced by TNF-α, inhibits OSCC cell proliferation and invasion via YAP phosphorylation.
  • LATS2 plays a critical role in OSCC tumorigenesis.
  • LATS2 represents a potential therapeutic target for OSCC treatment.
Keywords:
LATS2YAPoral squamous cell carcinomatumor necrosis factor-alpha

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