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Updated: Apr 16, 2026
Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
Published on: June 7, 2014
Methylene blue attenuates renal ischemia-reperfusion injury in rats
Fatma Sarac1, Huseyin Kilincaslan2, Elif Kilic3
1Department of Pediatric Surgery, Haseki Research and Education Hospital, Istanbul, Turkey.
Background And Purpose:
In our study, we investigated the effects of methylene blue (MB) on histopathological changes in renal ischemia/reperfusion (I/R) injury rat model.
Material And Methods:
Twenty-one Sprague-Dawley male rats were divided equally into three groups. Group 1 (control) was administered intraperitoneal saline solution. In Groups 2 (untreated group) and 3 (MB treatment), the renal arteries were clamped, and ischemia (for 1 hour) and then reperfusion (for 4 hours) were applied. Thirty minutes before ischemia, the untreated group received physiological saline, whereas the treatment group was administered 30 mg/kg MB through an intraperitoneal route. Blood samples were drawn, and renal specimens were harvested 5.5 hours after physiologic saline injection in the control and immediately after the reperfusion period in the other groups. The levels of tissue superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total oxidant status (TOS), total antioxidant status (TAS), plasma urea, creatinine and ischemia modified albumin (IMA) were measured. Moreover, the histopathological damage score of the renal tissue was determined.
Results:
MB significantly alleviated the severity of histopathological damage by increasing the levels of tissue SOD and TAS and decreasing TOS concentrations in the renal I/R model (p<0.05).
Conclusion:
Administration of MB in renal I/R damage may play a protective role.
Insights
Methylene blue (MB) treatment reduced kidney damage in a rat model of renal ischemia/reperfusion (I/R) injury. MB administration improved antioxidant levels and decreased oxidative stress, suggesting a protective role in I/R damage.
Area of Science:
- Nephrology
- Pharmacology
- Pathology
Background:
- Renal ischemia/reperfusion (I/R) injury is a significant clinical concern.
- Histopathological changes are a hallmark of renal I/R injury.
- Investigating novel therapeutic agents is crucial for mitigating I/R damage.
Purpose of the Study:
- To evaluate the therapeutic potential of methylene blue (MB) in a rat model of renal I/R injury.
- To assess the impact of MB on histopathological alterations in the kidneys.
- To determine the biochemical markers associated with oxidative stress and antioxidant status following I/R injury.
Main Methods:
- A rat model of renal I/R injury was established by clamping renal arteries for 1 hour followed by 4 hours of reperfusion.
- Rats were divided into control, untreated I/R, and MB-treated I/R groups.
- Methylene blue (30 mg/kg) was administered intraperitoneally 30 minutes before ischemia; biochemical markers and histopathological damage scores were assessed.
Main Results:
- Methylene blue significantly reduced the severity of histopathological damage in renal I/R injury.
- MB treatment led to increased levels of tissue superoxide dismutase (SOD) and total antioxidant status (TAS).
- MB administration decreased total oxidant status (TOS) in the renal I/R model (p<0.05).
Conclusions:
- Methylene blue demonstrates a protective effect against renal I/R injury.
- MB may exert its protective role by modulating oxidative stress and enhancing antioxidant defense mechanisms.
- Further research into MB as a therapeutic agent for renal I/R injury is warranted.
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