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Multi-spectroscopic methods investigation on the interaction of tenoxicam with DNA
Bing-Mi Liu1, Jun Zhang1, Yang Liu1
1Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
Abstract:
Non-steroidal anti-inflammatory drugs (NSAIDs) show chemopreventive and chemosuppressive effects on various cancer cell lines. They exert anticancer activities by inhibiting both at the protein level and/or at the transcription level. Thus, in this paper, the interaction between tenoxicam (TXM) and calf thymus DNA (ct-DNA) was investigated by UV-visible light, fluorescence, viscosity experiments and DNA melting studies. The results showed that TXM could bind to ct-DNA in the groove binding mode. The binding constants were 7.67 × 10(3) and 5.48 × 10(3) M(-1) at 293 and 300 K, respectively. Furthermore, the calculated thermodynamic parameters suggested that hydrogen bonds or van der Waals force might play an important role in the binding of TXM to ct-DNA. The obtained results should give new insight into the pharmacological activity of TXM.
Insights
Tenoxicam (TXM), a non-steroidal anti-inflammatory drug, binds to calf thymus DNA (ct-DNA) via groove binding. This interaction, driven by hydrogen bonds or van der Waals forces, offers insights into TXM's anticancer pharmacological activity.
Area of Science:
- Pharmacology
- Molecular Biology
- Biochemistry
Background:
- Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit chemopreventive and chemosuppressive properties against cancer cell lines.
- NSAIDs exert anticancer effects by inhibiting biological processes at both protein and transcriptional levels.
Purpose of the Study:
- To investigate the interaction mechanism between tenoxicam (TXM) and calf thymus DNA (ct-DNA).
- To elucidate the binding mode and thermodynamic parameters governing the TXM-ct-DNA interaction.
Main Methods:
- UV-visible spectrophotometry
- Fluorescence spectroscopy
- Viscosity measurements
- DNA melting studies
Main Results:
- Tenoxicam (TXM) binds to calf thymus DNA (ct-DNA) through groove binding.
- Binding constants were determined as 7.67 × 10^3 M⁻¹ at 293 K and 5.48 × 10^3 M⁻¹ at 300 K.
- Thermodynamic analysis indicated that hydrogen bonds and/or van der Waals forces are crucial for TXM-ct-DNA binding.
Conclusions:
- The study reveals the specific binding mode of tenoxicam to DNA.
- Findings provide a molecular basis for the pharmacological activity of TXM, particularly its potential anticancer effects.
