Related Experiment Video
Updated: Apr 16, 2026
06:03
Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
Published on: March 22, 2024
1.8K
Channel-based reporters for cAMP detection.
Thomas C Rich1, Wenkuan Xin, Silas J Leavesley
1Department of Pharmacology, University of South Alabama, MSB 3370, 5851 USA Drive North, Mobile, AL, 36688, USA, trich@southalabama.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2015
Summary
Researchers developed novel single-cell cyclic adenosine monophosphate (cAMP) sensors. These probes utilize cyclic nucleotide-gated (CNG) channels to detect cAMP levels near the cell membrane via ionic flux measurements.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Significant advancements in single-cell cyclic adenosine monophosphate (cAMP) sensors have occurred over the past 15 years.
- Existing sensors often rely on modified cAMP-binding proteins to convert cAMP concentrations into detectable electrical or fluorescent signals.
- These signals are typically measured using equipment such as patch clamp amplifiers, photomultiplier tubes, or cameras.
Purpose of the Study:
- To introduce two novel, complementary methods for detecting and quantifying cAMP signals specifically at the plasma membrane of cells.
- To leverage the unique properties of cyclic nucleotide-gated (CNG) channels for enhanced cAMP sensing.
Main Methods:
- Development of cAMP sensors based on cyclic nucleotide-gated (CNG) channels.
- Utilizing the ion-transducing capability of CNG channels to convert cAMP concentration changes into measurable ionic flux.
- Detection of cAMP levels through the measurement of Ca(2+) and/or Mn(2+) influx or ionic currents.
Main Results:
- The described probes effectively detect and measure cAMP signals localized near the plasma membrane.
- The use of CNG channels provides a sensitive mechanism for transducing cAMP fluctuations into ionic currents.
- The complementary approaches offer versatile tools for studying cellular cAMP dynamics.
Conclusions:
- The developed CNG channel-based probes represent a significant advancement in single-cell cAMP sensing technology.
- These methods offer precise spatial resolution for cAMP measurements at the cell membrane.
- The probes provide valuable tools for investigating the role of cAMP in cellular signaling pathways.
More Related Videos
Related Concept Videos
02:11Reporter Genes
13.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.9K
01:09Censoring Survival Data
687
Survival analysis is a statistical method used to analyze time-to-event data, often employed in fields such as medicine, engineering, and social sciences. One of the key challenges in survival analysis is dealing with incomplete data, a phenomenon known as "censoring." Censoring occurs when the event of interest (such as death, relapse, or system failure) has not occurred for some individuals by the end of the study period or is otherwise unobservable, and it might have many different...
687
01:21Types of Reports II: Incident or Occurrence Report
1.4K
An Incident or Occurrence Report in a healthcare setting is a crucial document used to record any unexpected occurrence that may or may not have affected a patient, employee, or visitor. Such reports are critical to improving patient safety and include all details leading up to and including the event.
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
1.4K
04:49Robbers Cave
15.2K
During the 1950s, the landmark Robbers Cave experiment demonstrated that when groups must compete with one another, intergroup conflict, hostility, and even violence may result. At the Oklahoman summer camp, two troops of boys—termed the Rattlers and the Eagles—took part in a week-long tournament. During this time, their negativity culminated in derogatory name-calling, fistfights, and even vandalism and destruction of property. However, this work also revealed that such tension...
15.2K
02:28Leaky Scanning
5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K
01:24Data Reporting and Recording
5.7K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.7K