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Updated: Apr 16, 2026

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

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Protein design: Past, present, and future.

Lynne Regan^1,2,3, Diego Caballero^3,4, Michael R Hinrichsen¹

¹Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT.

|March 19, 2015

Summary

This summary is machine-generated.

Protein design, pioneered in the 1980s, has evolved from understanding structure to creating novel proteins for practical applications. This review highlights key milestones, recent advances, and future directions in the field of protein engineering.

Keywords:

computation nanotechnology protein design review

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Area of Science:

Biochemistry
Structural Biology
Protein Engineering

Background:

The late 1980s marked a significant shift with pioneering protein design work by Ho and DeGrado.
Early protein design efforts significantly advanced the understanding of protein structure and stability.
Current research focuses on translating fundamental knowledge into practical protein applications.

Purpose of the Study:

To provide a historical overview of key milestones in protein design.
To highlight recent advancements and breakthroughs in the field.
To discuss future aspirations and potential applications of designed proteins.

Main Methods:

Review of seminal publications in protein design.
Analysis of historical trends and evolutionary pathways in protein engineering.
Synthesis of recent research findings and future outlooks.

Main Results:

Demonstration of protein design's evolution from basic science to applied engineering.
Identification of critical advancements that have shaped the field.
Outlining of emerging areas and future potential for novel protein creation.

Conclusions:

Protein design has transitioned from a fundamental science to an applied engineering discipline.
The field continues to advance, promising innovative solutions for diverse applications.
Future research will likely focus on expanding the capabilities and utility of designed proteins.