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Bioorthogonal deprotection on the dendritic cell surface for chemical control of antigen cross-presentation

Joanna B Pawlak1, Geoffroy P P Gential, Tracy J Ruckwardt

  • 1Leiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands).

Angewandte Chemie (International Ed. in English)
|March 19, 2015
PubMed

The activation of CD8(+) T-cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC-I complexes and presented to the T-cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated-antigens that are not recognized by their cognate T-cell unless they are deprotected on the cell using a Staudinger reduction.

Keywords:
Staudinger reductionantigensazidesbioorthogonal chemistrycell recognition

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