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Related Experiment Video

Updated: Apr 16, 2026

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

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Published on: August 9, 2024

Nanofiber adsorbents for high productivity continuous downstream processing.

Oliver Hardick¹, Stewart Dods¹, Bob Stevens²

¹Department of Biochemical Engineering, University College London, Torrington Place, WC1E 7JE London, United Kingdom; Micro and Nanotechnology Centre, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Didcot, OX11 0QX, United Kingdom.

Journal of Biotechnology

|March 19, 2015

Summary

This summary is machine-generated.

Continuous chromatography using cellulosic nanofibers and simulated moving bed (SMB) systems significantly boosts bioseparation productivity. This novel approach enhances efficiency and reduces costs in biotherapeutic manufacturing.

Keywords:

Anion exchange chromatography Diethylaminoethyl Nanofibers Simulated moving bed

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Area of Science:

Bioseparation and chromatography
Materials science for bioprocessing
Continuous manufacturing

Background:

Rising manufacturing costs of biotherapeutics necessitate innovative solutions.
Continuous processing offers advantages in cost reduction, process control, and integration.
Chromatographic operations can benefit from continuous processing for efficient media utilization.

Purpose of the Study:

To demonstrate the potential of cellulosic nanofibers as adsorbent materials in continuous chromatography.
To evaluate the performance of a high-frequency simulated moving bed (SMB) system utilizing nanofiber adsorbents.
To showcase enhanced productivity and efficiency in bioseparations using this novel system.

Main Methods:

Development and characterization of electrospun polymeric nanofiber adsorbents with anion exchange properties.
Implementation of a high-frequency simulated moving bed (SMB) chromatography system.
Performance evaluation based on flow properties, mass transfer, binding capacity, reproducibility, and lifecycle.

Main Results:

Cellulosic nanofiber adsorbents exhibited high convective mass transfer properties and rapid flow operations.
Reproducible bind-elute operations were achieved at flowrates of 2,400 cm/h, significantly exceeding conventional systems.
The SMB system with nanofiber adsorbents demonstrated a 15-fold increase in productivity compared to packed bed resins, with rapid 7s cycle times.

Conclusions:

Cellulosic nanofibers are highly effective adsorbent materials for continuous chromatography systems.
The combination of nanofiber adsorbents and SMB technology offers superior productivity in bioseparations.
This approach presents a significant advancement for cost-effective and efficient biotherapeutic manufacturing.