Related Experiment Video

Updated: Apr 16, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

9.7K

TET1-mediated different transcriptional regulation in prostate cancer

Jianhua Feng1, Qiang Wang2, Guangwei Li1

  • 1Urology Department of Longgang District Central Hospital Shenzhen City 518116, Guangdong Province, China.

International Journal of Clinical and Experimental Medicine
|March 19, 2015
PubMed

Insights

Prostate cancer (PCa) shows decreased 5-hydroxymethylcytosine (5 hmC) linked to Ten-eleven translocation (TET)1 reduction. TET1 influences gene expression via DNA methylation and hydroxymethylation, impacting PCa development.

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Global 5-hydroxymethylcytosine (5 hmC) levels are reduced in prostate cancer (PCa).
  • This decrease is associated with reduced activity of the DNA hydroxymethylase, Ten-eleven translocation (TET)1.
  • TET1 has a known dual role in epigenetic regulation, particularly in embryonic stem cells.

Purpose of the Study:

  • To elucidate the mechanism of DNA methylation and hydroxymethylation changes mediated by TET1 downregulation in PCa.
  • To investigate the specific roles of TET1 in regulating gene expression in prostate cancer.

Main Methods:

  • Bisulfite sequencing (BSP) was used to assess 5-methylcytosine (5 mC) levels in gene promoters.
  • Chromatin immunoprecipitation (ChIP) was employed to analyze H3K4me3 and H3K27me3 patterns in promoters.

Main Results:

  • Hypermethylation and reduced expression of Cdh1, Gstp1, Pten, Apc, Runx3, and Mgmt were observed in PCa.
  • Conversely, Cyr61, Sema3c, and Ptgs2 showed hypomethylation and increased expression in PCa.
  • Four marker genes exhibited Polycomb-repressed characteristics and were upregulated following TET1/PRC2 reduction in PCa.

Conclusions:

  • TET1 plays a distinct role in transcriptional regulation in prostate cancer.
  • TET1 mediates both activating and repressive functions, influencing gene expression patterns in PCa through epigenetic modifications.
Keywords:
5 hmC5 mCPRC2Prostate cancerTET1methylation

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.3K
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

25.2K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.3K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.5K