Superparamagnetic iron oxide promotes osteogenic differentiation of rat adipose-derived stem cells

Adult adipose tissue-derived stem cells (ADSCs) were found to hold great promise for use in bone tissue repair and regeneration. The present study aims to improve the osteogenesis of ADSCs by Superparamagnetic Iron Oxide (SPIO), which is widely used in tissue imaging. In this study, adipose-derived stem cells were harvested from 4-week-old male Sprague-Dawley (SD) rats. The proliferation rates of ADSCs labeling with or without SPIO were assessed by using trypan blue assay. The osteogenic capability was examined by employing the ALP activity detection kit. The mineralization of cells was determined by staining with Alizarin red S. Flow cytometry analysis was used to examine the cell apoptosis treated with or without SPIO. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis was utilized to detect the Runx2, Opn, Ocn and ALP genes in the cells. The results indicated that SPIO could promote rat ADSCs proliferation and reduce rat ADSCs apoptosis. Also, SPIO could significantly enhance the ALP and alizarin red staining of ADSCs in -SPIO group and +SPIO group (P < 0.01). Furthermore, we also found that the expression of Runx2, Opn, Ocn and ALP was significantly increased after SPIO treatment compared to the un-treated cells (P < 0.01). In conclusion, SPIO could promote the osteogenic differentiation of rat adipose-derived stem cells, which would also become a great potential therapeutic tool in bone tissue engineering.