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MC29 virus-coded protein occurs as monomers and dimers in transformed cells

J P Bader, D A Ray

    Journal of Virology
    |February 1, 1985
    PubMed

    Insights

    The MC29 virus protein p110gag-myc localizes to the nucleus and exists as monomers and dimers. This protein reassociates from monomeric forms, indicating dynamic structural behavior within cells.

    Area of Science:

    • Molecular Biology
    • Virology
    • Cell Biology

    Background:

    • The MC29 virus encodes the p110gag-myc protein.
    • Understanding the behavior of viral proteins is crucial for comprehending viral pathogenesis.

    Purpose of the Study:

    • To investigate the subcellular localization and oligomeric state of the MC29 virus-coded protein p110gag-myc.
    • To determine the stability and potential for reassociation of p110gag-myc.

    Main Methods:

    • Subcellular localization studies using transformed Japanese quail (Q8) cells.
    • Sucrose gradient sedimentation analysis of cell extracts under nondenaturing and denaturing conditions.
    • Enzyme treatments (RNase, DNase) and salt gradients to assess protein interactions.
    • Gel electrophoresis to detect protein reassociation.

    Main Results:

    • p110gag-myc was exclusively found in the nucleus of Q8 cells with a half-life of approximately 30 minutes.
    • Sedimentation analysis revealed p110gag-myc exists as monomers and dimers in nondenaturing conditions, and only as monomers under denaturing conditions.
    • High salt concentrations and enzyme treatments did not dissociate the faster-sedimenting forms, suggesting stable interactions.
    • Monomeric p110gag-myc demonstrated reassociation into dimers, observable by gel electrophoresis.

    Conclusions:

    • The MC29 virus protein p110gag-myc exhibits nuclear localization and exists in dynamic monomer-dimer equilibrium.
    • The protein's ability to reassociate from monomers to dimers suggests a self-assembly or interaction mechanism.
    • These findings contribute to understanding the molecular behavior of MC29 virus proteins within host cells.

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