Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.8K
MicroRNAs01:22

MicroRNAs

12.1K
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative Single-Cell and Bulk Transcriptomic Analysis Defines a Molecular Stratification Strategy Based on Intra-Tumoral Heterogeneity in Acral Melanoma.

Pigment cell & melanoma research·2026
Same author

Synergistic PdMoCu Trimetallic Metallene-Enhanced Electrochemiluminescence Biosensor for Ultrasensitive Detection of Microcystin-LR.

Biosensors·2026
Same author

The application of a new type of intestinal diversion devices in Beagle dogs with intestinal transection injury and hemorrhagic shock.

Frontiers in bioengineering and biotechnology·2026
Same author

Single-cell N4-acetylcytidine regulatory landscape identifies ARIH1 as a ferroptosis-suppressive oncogenic driver in lung adenocarcinoma.

Respiratory research·2026
Same author

Ethical decision-making confidence among nurse leaders: a mixed-methods study of determinants and ethical implications.

Frontiers in medicine·2026
Same author

Carrageenan/Poly(vinyl alcohol) Double-Network Hydrogel Incorporating Antioxidative Copper-l-Cysteine Nanoparticles for Infected Wound Treatment.

ACS biomaterials science & engineering·2026
Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026
Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026
Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026
Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026
Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026
Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K

Entropy-based model for miRNA isoform analysis.

Shengqin Wang1, Jing Tu2, Lei Wang2

  • 1State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.

Plos One
|March 19, 2015
PubMed
Summary
This summary is machine-generated.

MicroRNA isoforms (isomiRs) exhibit significant variations, particularly at the 5p end, influencing gene expression. These variations are linked to pre-miRNA features and critical biological functions, revealing novel regulatory insights.

More Related Videos

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

8.0K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

523

Related Experiment Videos

Last Updated: Apr 16, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

8.0K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

523

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression.
  • miRNA isoforms (isomiRs) are increasingly detected in sequencing data, but their functions remain largely unexplored.

Purpose of the Study:

  • To develop a computational model for analyzing isomiR expression profiles.
  • To investigate the biological significance and regulatory factors of isomiR variations.

Main Methods:

  • Developed a Shannon entropy-based model to estimate isomiR expression.
  • Utilized the Kolmogorov-Smirnov (KS) statistical test for variant analysis.
  • Performed functional enrichment analysis on miRNAs with high variation.

Main Results:

  • Identified that 5p and 3p miRNAs exhibit more variants than single-arm miRNAs.
  • Found a strong correlation between isomiR variants (excluding 3' variants) and pre-miRNA Minimum Free Energy (MFE).
  • Demonstrated that highly varied miRNAs, especially 5' end variations, are enriched in critical cellular functions.

Conclusions:

  • Established a probabilistic framework for miRNA isoform analysis.
  • Provided functional insights suggesting isomiRs are not randomly produced and are influenced by pre-miRNA processing.
  • Highlighted the importance of intrinsic pre-miRNA features in miRNA regulation.