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The effects of kisspeptin-10 on migration and proliferation of endothelial cell
Fatemeh Golzar1, Shaghayegh Haghjooy Javanmard1
1Department of Physiology, Physiology Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
Background:
Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade include invasion, migration and homing, angiogenesis, survival and proliferation. In this study we investigated effects of kisspeptin-10, the most potent member of kisspeptin family, on Migration and proliferation of endothelial cells that are necessary for angiogenesis and tumor metastasis.
Materials And Methods:
We compared migration of Human Umbilical Vein Endothelial Cells (HUVECs) were treated with 10-100 or 500 nM kp-10 for 24 hours and no treated cells using an in vitro trans membrane migration assay and HUVEC proliferation of treated endothelial cells with 10-100 or 500 nM kp-10 for 48 hours and no treated cells was measured by MTT Cell Proliferation Assay Kit. Analysis of data was performed using the Kruskal-Wallis test followed by the Mann-Whitney test.
Results:
Migration and proliferation of endothelial cells were increased at lower concentration of kp-10 specially at 100 nM while higher concentration reduced both migration and proliferation.
Conclusion:
Our data showed that different concentrations of kp-10 have distinct effects on migration and proliferation of endothelial cells.
Insights
Kisspeptin-10 (kp-10) impacts endothelial cell migration and proliferation, crucial for tumor growth. Lower kp-10 concentrations enhance these processes, while higher concentrations inhibit them, suggesting a dose-dependent effect.
Area of Science:
- Endocrinology
- Cell Biology
- Oncology
Background:
- Endothelial cell migration, expansion, and survival are critical for tumor angiogenesis and metastasis.
- Kisspeptins (kp), acting via GPR54, are known to inhibit various stages of the metastatic cascade.
- Kisspeptin-10 (kp-10) is the most potent member of the kisspeptin family.
Purpose of the Study:
- To investigate the effects of kp-10 on endothelial cell migration and proliferation.
- To determine the role of kp-10 in processes essential for angiogenesis and tumor metastasis.
Main Methods:
- Human Umbilical Vein Endothelial Cells (HUVECs) were treated with varying concentrations of kp-10 (10-500 nM).
- Cell migration was assessed using an in vitro transwell migration assay.
- Cell proliferation was measured using the MTT Cell Proliferation Assay Kit.
Main Results:
- Lower concentrations of kp-10, particularly 100 nM, significantly increased endothelial cell migration and proliferation.
- Higher concentrations of kp-10 (e.g., 500 nM) demonstrated an inhibitory effect on both migration and proliferation.
- Statistical analysis involved the Kruskal-Wallis and Mann-Whitney tests.
Conclusions:
- Different concentrations of kp-10 exert distinct and opposing effects on endothelial cell migration and proliferation.
- These findings highlight a complex, dose-dependent role for kp-10 in regulating angiogenesis and tumor metastasis.
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