Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

In vitro Mutagenesis

In vitro Mutagenesis

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neuropeptides Are Involved in Elicited Reversal Speed Plasticity in <i>C. elegans</i> During Mechanosensory Habituation.

microPublication biology·2026

Same author

Neuropeptides Involved in Elicited Reversal Speed Plasticity in <i>C. elegans</i> During Mechanosensory Habituation.

bioRxiv : the preprint server for biology·2025

Same author

Ethanol alters mechanosensory habituation in C. elegans by way of the BK potassium channel through a novel mechanism.

PloS one·2025

Same author

A familial Alzheimer's disease associated mutation in presenilin-1 mediates amyloid-beta independent cell specific neurodegeneration.

PloS one·2024

Same author

wrk-1 and rig-5 control pioneer and follower axon navigation in the ventral nerve cord of Caenorhabditis elegans in a nid-1 mutant background.

Genetics·2022

Same author

Stereotyped behavioral maturation and rhythmic quiescence in <i>C. elegans</i> embryos.

eLife·2022

Same journal

Fast-conducting mechanonociceptors uniquely engage reflexive and affective pain circuitry to drive protective responses.

Neuron·2026

Same journal

Sparse component analysis: A method that uncovers separable computations within neural population activity.

Neuron·2026

Same journal

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens.

Neuron·2026

Same journal

TGF-β1-induced endothelial transcytosis drives blood-brain barrier leakage during aging.

Neuron·2026

Same journal

Image space opens up for visual neuroscience.

Neuron·2026

Same journal

Septal GLP-1 receptors control alcohol taking and seeking.

Neuron·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 16, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Casting a genome-wide net for learning mutants.

Evan L Ardiel¹, Catharine H Rankin²

¹Brain Research Centre, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada.

|March 20, 2015

Summary

This summary is machine-generated.

Pregnancy-associated plasma protein-AA (PAPP-AA) plays a key role in habituation of the acoustic startle response in zebrafish. This protein regulates this learning process through insulin-like growth factor (IGF) signaling.

More Related Videos

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

Related Experiment Videos

Last Updated: Apr 16, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

Area of Science:

Neuroscience
Genetics
Developmental Biology

Background:

Habituation is a fundamental form of learning.
The acoustic startle response is a widely studied reflex.
Genetic screens are powerful tools for identifying molecular mechanisms.

Purpose of the Study:

To identify genetic factors regulating habituation of the acoustic startle response in zebrafish.
To elucidate the molecular pathways involved in this form of learning.

Main Methods:

Forward genetic screen in zebrafish.
Analysis of gene expression patterns.
Investigating signaling pathways.

Main Results:

A forward genetic screen identified pregnancy-associated plasma protein-AA (PAPP-AA) as a key regulator of acoustic startle response habituation.
PAPP-AA is expressed in relevant neural circuits, including Mauthner cells.
PAPP-AA mediates habituation through insulin-like growth factor (IGF) signaling.

Conclusions:

PAPP-AA is a novel molecular player in the neural basis of habituation.
IGF signaling is implicated in the regulation of learning and memory processes.