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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

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In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
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Replication in Eukaryotes01:29

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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Replication in Eukaryotes02:31

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Replicative Cell Senescence02:15

Replicative Cell Senescence

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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Related Experiment Video

Updated: Apr 16, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
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In vitro Reconstitution of the Active T. castaneum Telomerase

Published on: July 14, 2011

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Telomerase redux: ready for prime time?

James W Larrick1, Andrew R Mendelsohn

  • 1Panorama Research Institute and Regenerative Sciences Institute , Sunnyvale, California.

Rejuvenation Research
|March 20, 2015
PubMed
Summary

Telomerase, crucial for genome stability and aging, has two components: telomerase RNA (TERC) and telomerase reverse transcriptase (TERT). AAV-expressed TERT may extend lifespan, but its tightly regulated synthesis is vital due to cancer risks.

Area of Science:

  • Molecular Biology
  • Genetics
  • Gerontology

Background:

  • Telomerase maintains genome integrity, cell proliferation, and tissue homeostasis, impacting aging and cancer.
  • Telomerase comprises telomerase RNA (TERC) as a template and telomerase reverse transcriptase (TERT) as the catalytic subunit.
  • TERT synthesizes telomeric DNA and maintains telomere length, crucial for cellular function.

Purpose of the Study:

  • To explore the role of telomerase reverse transcriptase (TERT) in aging and cancer.
  • To investigate the potential of adeno-associated virus (AAV)-expressed TERT for increasing lifespan and healthspan.
  • To address the need for regulated TERT synthesis due to its association with oncogenic transformation.

Main Methods:

  • Review of recent studies on TERT function and expression.

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
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  • Analysis of TERT's interaction with gene-regulating proteins.
  • Consideration of safety concerns regarding TERT expression levels.

    • Main Results:

    • TERT is essential for telomere maintenance and has implications in aging and cancer.
    • AAV-mediated TERT expression in mice suggests potential for life extension and improved healthspan.
    • High TERT levels are linked to oncogenic transformation, necessitating careful regulation.

    Conclusions:

    • TERT plays a dual role in cellular health and disease, particularly in aging and cancer.
    • Transient TERT mRNA expression is a potentially safer approach for therapeutic applications like tissue engineering and stem cell therapy.
    • Controlled TERT synthesis is critical for harnessing its benefits while mitigating oncogenic risks.