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This study designs a luminescent compound that uses two stages of sensitizers to provide consistent signals across varying concentrations. This approach enables reliable monitoring in complex bioassays by combining aggregation-caused quenching and aggregation-induced emission effects.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Aggregation-caused quenching (ACQ) and aggregation-induced emission (AIE) are crucial phenomena in luminescence.
  • Designing luminescent probes with consistent signals across concentration ranges is challenging for bioassays.

Purpose of the Study:

  • To design a luminescent compound with consistent emission signals across a wide concentration range.
  • To develop a smart lanthanide bioprobe applicable to complex bioassay systems with variable concentrations.

Main Methods:

  • Utilized two stages of sensitizers incorporating both ACQ and AIE effects.
  • Stimulated lanthanide emission below 10(-4) M via ACQ and above 10(-3) M via AIE.
  • Monitored molecular concentration digitally using maximal excitation wavelengths and their linear relationship with concentration {lg(M)}.

Main Results:

  • Achieved consistent luminescent emission signals across a broad concentration spectrum.
  • Demonstrated a linear relationship between maximal excitation wavelengths and molecular concentration.
  • Successfully designed a dual-stage sensitizer system for lanthanide emission.

Conclusions:

  • The developed model provides a practical strategy for designing smart lanthanide bioprobes.
  • The dual-stage sensitizer system (AIE and ACQ effects) is suitable for variable concentration bioassays.
  • This approach offers a novel method for digital concentration monitoring in complex biological systems.