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Related Concept Videos

RNA-seq03:21

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Quantification and quality control of a small non-coding RNA preparation.

Virginie Marchand1, Christiane Branlant

  • 1Université de Lorraine, CNRS UMR 7365 IMoPA, Vandoeuvre-lès-Nancy, France, virginie.marchand@univ-lorraine.fr.

Methods in Molecular Biology (Clifton, N.J.)
|March 21, 2015
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Summary

High-throughput sequencing reveals diverse small non-coding RNAs (sncRNAs) involved in gene regulation and disease. This chapter details gold standard methods for accurate sncRNA quantification and integrity control for reliable downstream analysis.

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • High-throughput sequencing technologies have expanded the understanding of small non-coding RNA (sncRNA) diversity.
  • sncRNAs play critical roles in regulating gene expression and are implicated in various diseases.
  • Accurate sncRNA profiling is essential for both research and clinical applications.

Purpose of the Study:

  • To describe gold standard methods for RNA quantification in sncRNA profiling.
  • To outline techniques for controlling RNA integrity prior to downstream applications.
  • To provide a comprehensive guide for reliable sncRNA analysis.

Main Methods:

  • Detailed description of established RNA quantification techniques.
  • Explanation of methods for assessing and ensuring RNA integrity.
  • Discussion of best practices for sample preparation in sncRNA studies.

Main Results:

  • The chapter provides a review of validated methods for sncRNA quantification.
  • It highlights critical steps for maintaining RNA integrity.
  • It emphasizes the importance of these steps for downstream experimental success.

Conclusions:

  • Accurate quantification and integrity control are foundational for robust sncRNA profiling.
  • Adherence to gold standard methods ensures reliable data for gene regulation and disease research.
  • This chapter serves as a key resource for researchers in the field of sncRNA analysis.