Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.1K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.1K
RNA Interference01:23

RNA Interference

28.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.9K
MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.8K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

5.4K
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Addressing the needs of nano-rare patients: the n-Lorem experience.

Nucleic acids research·2026
Same author

Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products.

Nucleic acids research·2026
Same author

A missense mutation in the SAA1 protein causing hereditary amyloid A amyloidosis.

Kidney international·2026
Same author

Establishing a commercial solution for extremely rare genetic diseases.

Nature biotechnology·2026
Same author

<i>Letter:</i> Correspondence on Damle, S.S., <i>et al.</i> (2025) A Workflow for Transcriptome-Wide Assessment of Antisense Oligonucleotide Selectivity. Nucleic Acid Therapeutics.

Nucleic acid therapeutics·2025
Same author

Multi-Parametric MRI for Early Detection of Renal Fibrosis and Evaluation of Therapeutic Effect of Asiatic Acid in an Experimental Rat.

NMR in biomedicine·2025

Related Experiment Video

Updated: Apr 16, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

8.4K

Efficient and selective knockdown of small non-coding RNAs.

Xue-Hai Liang1, Wen Shen, Stanley T Crooke

  • 1Department of Core Antisense Research, ISIS Pharmaceuticals Inc., Carlsbad, CA, 92010, USA, LLiang@isisph.com.

Methods in Molecular Biology (Clifton, N.J.)
|March 21, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to deplete small non-coding RNAs (ncRNAs) using antisense oligonucleotides and RNase H. This technique aids in studying ncRNA functions by enabling selective reduction of these molecules in human cells.

More Related Videos

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

11.5K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K

Related Experiment Videos

Last Updated: Apr 16, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

8.4K
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

11.5K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Small non-coding RNAs (ncRNAs) are crucial regulators of gene expression and other biological processes.
  • Characterizing small ncRNAs is challenging due to difficulties in their selective depletion.
  • Existing methods often lack specificity or efficiency for small ncRNA reduction.

Purpose of the Study:

  • To present a novel protocol for the selective depletion of small non-coding RNAs (ncRNAs) in human cells.
  • To overcome methodological obstacles in small ncRNA characterization.
  • To enable functional studies of small ncRNAs through targeted degradation.

Main Methods:

  • Utilizing second-generation antisense oligonucleotides (ASOs) designed for specific small ncRNA sequences.
  • Employing the RNase H cleavage pathway to degrade targeted RNAs.
  • Implementing a protocol involving oligonucleotide design, cell transfection, RNA extraction, and detection.

Main Results:

  • Demonstrated successful depletion of small ncRNAs using the ASO-directed RNase H approach.
  • Showcased the versatility of the method for various small ncRNA types.
  • Established a reliable protocol for researchers to reduce specific small ncRNAs.

Conclusions:

  • The described antisense oligonucleotide-directed RNase H cleavage pathway offers an effective strategy for small ncRNA depletion.
  • This method facilitates the functional characterization of small ncRNAs by enabling their selective reduction.
  • The protocol provides a valuable tool for molecular biology and gene expression research.