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A simple and robust method for pre-wetting poly (lactic-co-glycolic) acid microspheres.

Bernice Wright¹, Nina Parmar¹, Laurent Bozec²

¹Applied Biomedical Engineering Group, Division of Medicine, University College London.

Journal of Biomaterials Applications

|March 21, 2015

Summary

A new one-step ethanol immersion method efficiently wets poly (lactic-co-glycolic) acid microspheres for improved cell delivery. This protocol enhances myoblast attachment by altering surface properties and protein adsorption.

Keywords:

Poly (lactic-co-glycolic) acid microspheres human skeletal myoblasts and thermally induced phase separation microspheres poly (lactic-co-glycolic) acid microsphere surface topography serum protein adsorption thermally induced phase separation thermally induced phase separation microspheres wetting poly (lactic-co-glycolic) acid microspheres

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Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Poly (lactic-co-glycolic) acid (PLGA) microspheres are versatile for biomedical delivery.
Effective cell attachment to PLGA requires proper surface wetting (hydrophilisation).
Conventional wetting methods are often multi-step and time-consuming.

Purpose of the Study:

To develop a simplified, efficient one-step method for wetting PLGA microspheres.
To optimize PLGA microsphere wetting for enhanced cell delivery applications.
To investigate the impact of wetting on myoblast attachment and surface properties.

Main Methods:

A novel one-step ethanol immersion protocol for PLGA microsphere wetting.
Evaluation of wetting efficiency using varying ethanol concentrations (35%, 70%, 100%).
Assessment of human skeletal myoblast attachment to wetted microspheres.
Analysis of surface topography and serum protein adsorption.

Main Results:

The one-step ethanol immersion method significantly improved PLGA microsphere wetting.
70% (v/v) ethanol facilitated efficient wetting and superior myoblast attachment compared to 35% (v/v) ethanol.
Ethanol pre-wetting altered microsphere surface topography, creating a more rugose surface.
Increased serum protein adsorption was observed on microspheres pre-wetted with 70% ethanol, promoting myoblast adhesion.

Conclusions:

The developed one-step ethanol immersion protocol is an efficient method for wetting PLGA microspheres.
This protocol enhances myoblast delivery by improving cell attachment through biochemical and structural modifications.
The findings facilitate the use of PLGA microspheres in clinical settings for cell-based therapies.