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Related Experiment Video

Updated: Apr 16, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
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Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

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Harvesting technique affects adipose-derived stem cell yield.

Tejaswi Iyyanki1, Justin Hubenak1, Jun Liu1

  • 1From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Aesthetic Surgery Journal
|March 21, 2015
PubMed
Summary
This summary is machine-generated.

Direct excision or Coleman

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Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Autologous fat grafting success relies on stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs).
  • Harvesting techniques and donor sites significantly impact SVF and ASC yields.
  • Understanding these variations is crucial for optimizing fat graft outcomes.

Purpose of the Study:

  • To evaluate how adipose tissue harvesting methods and donor locations influence the quantity of ASCs and SVF cells obtained.
  • To identify optimal techniques for maximizing stem cell yield in fat grafts.

Main Methods:

  • Subcutaneous fat was harvested from abdomen, flank, or axilla using liposuction, direct excision, or Coleman's technique.
  • Cells were isolated and quantified via flow cytometry for SVF and ASC yields.
  • Statistical analysis (ANOVA) compared yields across techniques and sites.

Main Results:

  • Direct excision yielded significantly more ASCs and SVF cells compared to Coleman's technique without centrifugation.
  • Liposuction resulted in significantly lower ASC and SVF cell yields.
  • Abdominal fat yielded more SVF cells than flank or axilla fat.
  • Coleman's technique with centrifugation increased SVF cell yield but not ASC yield.

Conclusions:

  • Abdominal fat harvested via direct excision or Coleman's technique with centrifugation provides the highest yields of SVF cells and ASCs.
  • These findings can guide clinical practices to improve autologous fat grafting efficacy.