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Cell purification: a new challenge for biobanks.

Maria Almeida, Andres C García-Montero, Alberto Orfao

    Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
    |March 21, 2015
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    Summary

    Purifying cells from complex samples like blood is crucial for accurate

    Area of Science:

    • Biotechnology
    • Genomics
    • Proteomics

    Background:

    • Heterogeneous biological samples can yield biased '-omics' data.
    • Low-abundance cells/molecules are masked in whole-sample analyses.
    • High-quality purified cells are vital for detecting subtle biological variations.

    Purpose of the Study:

    • Highlight the importance of cell purification in biobanks.
    • Review current and emerging cell sorting techniques for biobanking.
    • Discuss technical considerations for high-throughput cell sorting in biobanks.

    Main Methods:

    • Review of existing cell purification techniques (adherence, density, size, antibody-based, lab-on-a-chip).
    • Analysis of technical advantages and limitations of each method.

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  • Discussion of requirements for high-throughput (HT) cell sorting in biobanks.

    • Main Results:

    • Cell purification is critical for reliable '-omics' analysis.
    • Various cell purification methods exist, each with pros and cons.
    • Current methods often lack high-throughput capabilities for biobanks.

    Conclusions:

    • Preparative cell sorting offers significant opportunities for biobanks.
    • Selecting the right purification technology depends on recovery, purity, and yield.
    • Development of HT cell sorting is needed to meet biobank demands.