Immunohistochemical analysis of human MHC class II antigens in B-cell non-Hodgkin's lymphomas

Twenty cases of B-cell non-Hodgkin's lymphoma (NHL) were evaluated by immunohistochemical staining of frozen sections with a panel of anti MHC class II monoclonal antibodies (MCA). These studies showed marked differences in the expression of class II antigens both between different cases and within the population of cells of individual cases. In all of the cases studied the majority of the tumour cells reacted with MCAs directed against determinants common to the products of SB and DR loci. However, MCAs specific for DC determinants failed to react with 3/20 cases and in several other cases stained only a minority of cells. Absent or reduced expression of DC antigens was most marked in lymphocytic lymphoma; however, in centroblastic-centrocytic and centroblastic lymphomas, DC antigens could be detected on the majority of cells.