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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Sphingobacterium yamdrokense sp. nov., isolated from Lake Yamdrok
Na Xiao1, Yongqin Liu, Zhengquan Gu
1Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, People's Republic of China, xiaona@itpcas.ac.cn.
Abstract:
A strictly aerobic and facultatively psychrophilic bacterium, strain 3-0-1(T), was isolated from Lake Yamdrok on the Tibetan Plateau, China. Cells were Gram-negative and short-rod-shaped. Strain 3-0-1(T) formed circular, opaque, yellow colonies, and grew at 0-5% (w/v) NaCl, pH 5.0-11.0 and 5-35 °C. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain 3-0-1(T) belongs to the genus Sphingobacterium, family Sphingobacteriaceae, sharing the highest similarity with Sphingobacterim nematocida M-SX103(T) (96.62%) and less than 95% similarity with all the other species in the genus Sphingobactrium. The predominant isoprenoid quinone of strain 3-0-1(T) was identified as menaquinone 7 (MK-7), the major polar lipid was identified as phosphatidylethanolamine and the DNA G+C content was determined to be 42.5 mol%. The predominant cellular fatty acids were identified as summed feature 3 (C(16:1) ω7c and/or C(16:1) ω6c) (>30%), iso-C(15:0) (>20%) and iso-C(17:0) 3-OH (>10%). Based on the variability of phylogenetic and phenotypic characteristics, strain 3-0-1(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium yamdrokense sp. nov. is proposed. The type strain is 3-0-1(T) (=JCM 19397(T) = CGMCC 1.12560(T)).
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