Biomedical molecular of woody extractives of Cunninghamia Lanceolata biomass

Extractives, important compounds from wood, provide abundant resources for woody medicine. In this study, the three extractives from Cunninghamia lanceolata wood were removed by method of three-stage extraction with alcohol, petroleum ether, and alcohol/petroleum ether and their chemical components were analyzed by gas chromatography-mass spectrometry (GC-MS). Thirteen chemical components were discovered in the first-stage extractives, including: 4-((1e)-3-hydroxy-1-propenyl)-2-methoxyphenol (36.80%), α-(2-phenylethenyl)-1-piperidineacetonitrile (15.39%). One-hundred chemical components were discovered in the second-stage extractives, including: [1s-(1α,4aα,10aβ)]-1, 2,3,4,4a,9,10,10a-octahydro-1,4a- dimethyl-7-(1-methylethyl)-1- phenanthrenecar-boxylic acid (15.16%), 1,3-dimethoxy-5-[(1e)-2- phenylethenyl]-benzene (6.99%). Seven chemical components were discovered in the third-stage extractives, including: 1,3-dimethoxy -5-[(1E)-2-phenylethenyl]-benzene (32.88%), stigmasta-4,6,22-trien-3α-ol (17.83%). And both the main retention time of the first-stage and which of third-stage extractives are 20-30 minutes, and the main retention time of the second-stage extractives is <10 minutes. Besides, the three extractives contained many biomedical molecular, such as [1s-(1α,4aα,10aβ)]-1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-(1-methylethyl)-1-phenanthrenecar-boxylic acid, squalene, stigmast-4-en-3-one and γ-sitosterol and so on, which means that the three extractives from Cunninghamia lanceolata wood have huge potential in biomedicine.