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Updated: Apr 15, 2026

Characterizing Electron Transport through Living Biofilms
Published on: June 1, 2018
Reductive behaviour of acid azo dye based wastewater: Biocatalyst activity in conjunction with enzymatic and
S Sreelatha1, C Nagendranatha Reddy1, G Velvizhi1
1Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
Abstract:
Present study illustrates the significance of biocatalyst's reductive behaviour in the degradation of dye molecules using glucose as co-substrate. An anaerobic system was operated at a dye concentration of 50mg/l with an organic loading rate (OLR) of 1.36 kg COD/m(3)-day. Decolourization and COD removal efficiencies were observed to be 42% and 48% respectively. Azo reductase (18.9 U) and dehydrogenase enzyme (1.4 μg/ml) activities showed increment with operation time. Anaerobic microenvironment showed dye reduction converting them into aromatic amines. The presence of mediators viz., cytochromes, quinines and Fe-S proteins depicted in the cyclic voltammetry profiles played a crucial role in transfer of electrons for the reduction of dye molecules. Bio-electro kinetic profiles obtained through Tafel analysis showed persistent reduction behaviour, which is in good correlation with dye degradation in the anaerobic microenvironment.
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