A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide
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Updated: Apr 15, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
Kyoungmin Roh1, Dong-Min Kim, Eun Hee Lee
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
Abstract:
We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5'→ 3' exonuclease activity of Taq polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.
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