Next-generation Raman tomography instrument for non-invasive in vivo bone imaging
Jennifer-Lynn H Demers1, Francis W L Esmonde-White2, Karen A Esmonde-White3
1Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, 03755, USA ; JLHD and FEW have made equal contributions to the manuscript.
Abstract:
Combining diffuse optical tomography methods with Raman spectroscopy of tissue provides the ability for in vivo measurements of chemical and molecular characteristics, which have the potential for being useful in diagnostic imaging. In this study a system for Raman tomography was developed and tested. A third generation microCT coupled system was developed to combine 10 detection fibers and 5 excitation fibers with laser line filtering and a Cytop reference signal. Phantom measurements of hydroxyapatite concentrations from 50 to 300 mg/ml had a linear response. Fiber placement and experiment design was optimized using cadaver animals with live animal measurements acquired to validate the systems capabilities. Promising results from the initial animal experiments presented here, pave the way for a study of longitudinal measurements during fracture healing and the scaling of the Raman tomography system towards human measurements.
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