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Abl1 inhibitory contaminants leach from plastic tubes
Hui Chen1, Bart Blanchaert1, Abhijit Babaji Shinde2
1a Pharmaceutical Analysis , Faculty of Pharmaceutical Sciences , KU Leuven, Leuven , Belgium .
Journal of Enzyme Inhibition and Medicinal Chemistry
|March 24, 2015
Summary
Disposable plastic tubes used in labs can release contaminants that inhibit Abl1 activity. These bioactive leachates show no significant cytotoxicity, suggesting they may be specific tyrosine kinase inhibitors.
Area of Science:
- Biochemistry
- Materials Science
- Cell Biology
Background:
- Disposable plasticware is essential for life science research, enabling sample handling.
- Emerging evidence indicates that some plastic labware can release bioactive contaminants.
- These contaminants may interfere with biological processes and experimental outcomes.
Purpose of the Study:
- To investigate the potential interference of bioactive leachates from plastic tubes with Abl1 activity.
- To characterize the inhibitory effects and cytotoxicity of these leachates.
- To determine if plastic tube leachates act as specific inhibitors of tyrosine kinases.
Main Methods:
- Plastic tubes were used as incubators for Abl1.
- Bioactive leachates were extracted from the plastic tubes.
- Inhibitory activity against Abl1 and cytotoxicity of the extracts were assessed.
Main Results:
- Extracts from the plastic tubes demonstrated inhibitory activity against Abl1.
- No significant cytotoxicity was observed from the tube extracts.
- The leachates interfered with the catalytic activity of Abl1.
Conclusions:
- Bioactive leachates from disposable plastic tubes can inhibit Abl1 activity.
- These leachates do not exhibit significant cytotoxicity.
- Plastic tube leachates may function as specific inhibitors of tyrosine kinase activity, impacting research findings.
