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Oligosaccharide-dependent and independent Qa-1 determinants
Journal of Immunology (Baltimore, Md. : 1950)
|May 1, 1985
Summary
N-linked oligosaccharides stabilize some Qa-1 determinants, but others remain intact without them. This study used cloned cytotoxic T lymphocytes (CTL) and tunicamycin to investigate glycosylation
Area of Science:
- Immunology
- Molecular Biology
- Glycobiology
Background:
- The role of N-linked oligosaccharides in forming determinants recognized by alloreactive cytotoxic T lymphocytes (CTL) has not been established.
- Most studies indicate that allodeterminants on class I molecules are not glycosylation-dependent for serologic detection.
Purpose of the Study:
- To investigate the contribution of N-linked oligosaccharides to the formation of Qa-1 determinants recognized by cloned CTL.
- To determine whether carbohydrate addition is essential for the expression of specific Qa-1 determinants.
Main Methods:
- Utilized cloned cytotoxic T lymphocytes (CTL) and tunicamycin, a protein glycosylation inhibitor.
- Examined tunicamycin-treated, Concanavalin A-activated lymphoblasts from various Qa-1 genotypes.
- Correlated CTL clone reactivity with target cells based on glycosylation dependence of Qa-1 determinants.
Main Results:
- Carbohydrate addition was required for two of six identified Qa-1 determinants.
- Other Qa-1 determinants were detectable on nonglycosylated molecules.
- CTL clones exhibited specific recognition patterns: some dependent on glycosylation, others independent, indicating recognition of conformational determinants.
Conclusions:
- N-linked oligosaccharides stabilize the conformation of certain Qa-1 determinants.
- Some Qa-1 determinants remain intact and recognizable on nonglycosylated molecules.
- The findings suggest Qa-1 molecules may possess unique glycosylation-dependent recognition properties compared to other class I antigens, warranting further investigation.