Measuring Rab GTPase-activating protein (GAP) activity in live cells and extracts

Ryan M Nottingham1, Suzanne R Pfeffer

  • 1Department of Biochemistry, Stanford University School of Medicine, 291 Campus Drive, Stanford, CA, 94305-5307, USA.

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Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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