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The effects of adriamycin on E-cadherin mediated cell-cell adhesion and apoptosis during early kidney development

A Yay1, S Ozdamar, E Balcioglu

  • 1Department of Histology and Embryology, University of Erciyes, Medicine Faculty , Kayseri , Turkey.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|March 25, 2015
PubMed

Insights

Adriamycin (ADR) causes kidney damage and apoptosis in developing rat embryos. This study found increased E-cadherin expression linked to ADR-induced nephrotoxicity.

Area of Science:

  • Developmental toxicology
  • Renal pathology
  • Cell biology

Background:

  • Adriamycin (ADR) is a known teratogen with potential developmental toxicity.
  • Understanding ADR's impact on organogenesis is crucial for mitigating adverse effects.

Purpose of the Study:

  • To investigate the effects of Adriamycin on apoptosis and E-cadherin expression in developing rat kidneys.
  • To elucidate the mechanisms underlying ADR-induced nephrotoxicity during development.

Main Methods:

  • Pregnant rats received ADR or saline during gestation.
  • Embryonic kidneys were collected at various developmental stages (days 13, 15, 17, 19).
  • Histological, immunohistochemical (E-cadherin), and TUNEL assays were performed.

Main Results:

  • ADR exposure led to kidney damage, including tubule and glomerular alterations.
  • Increased apoptosis was observed in ADR-exposed kidney tissues.
  • E-cadherin expression was significantly upregulated in developing kidneys exposed to ADR.

Conclusions:

  • ADR induces apoptosis and alters E-cadherin expression in the developing rat kidney.
  • These changes, including increased E-cadherin, may contribute to Adriamycin's nephrotoxicity.
Keywords:
E-cadherinadriamycinapoptosiskidney development

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