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Updated: Apr 15, 2026

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A vanG-type locus in Clostridium argentinense.

Nicolas Berthet1, Bruno Périchon2, Christelle Mazuet3

  • 1Centre National de la Recherche Scientifique, UMR3569 Paris, France Centre International de la Recherche Médicale de Franceville, Unité des Zoonoses et Maladies Emergentes, Franceville, Gabon.

The Journal of Antimicrobial Chemotherapy
|March 25, 2015
PubMed
Summary

A new vanGCar gene locus in Clostridium argentinense is cryptic and intrinsic. Decryptification of this vanGCar gene cluster could lead to vancomycin resistance emergence.

Keywords:
C. argentinenseresistancevancomycin

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Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Clostridium argentinense harbors a novel vanG-type locus, designated vanGCar.
  • Understanding the function and regulation of vanGCar is crucial for predicting antimicrobial resistance.
  • Glycopeptide resistance mechanisms in Gram-positive bacteria are a significant public health concern.

Purpose of the Study:

  • To investigate the novel vanGCar locus in Clostridium argentinense.
  • To determine the impact of the vanGCar locus on glycopeptide susceptibility.
  • To elucidate the expression and regulation of the vanGCar genes.

Main Methods:

  • Whole genome sequencing of C. argentinense NCIB 10714 using Illumina technology.
  • Polymerase Chain Reaction (PCR) to detect vanGCar presence in seven strains.
  • Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) to assess vanGCar expression.
  • Etest procedure to determine glycopeptide susceptibility.

Main Results:

  • The vanGCar locus, containing genes for carboxypeptidase, D-alanine:D-serine ligase, serine transporter, and serine racemase, was identified in all seven studied C. argentinense strains.
  • An AraC-type transcriptional regulator was found upstream of the vanGCar genes.
  • C. argentinense NCIB 10714 remained susceptible to vancomycin and teicoplanin.
  • qRT-PCR showed no vanGCar expression under basal conditions or upon induction with subinhibitory vancomycin concentrations.

Conclusions:

  • The vanGCar locus in C. argentinense is cryptic and intrinsic to the species.
  • The potential for vancomycin resistance emergence exists through the decryptification of the vanGCar gene cluster.
  • Further research is needed to understand the regulatory mechanisms controlling vanGCar expression.